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primar antibodies against myeloperoxidase  (Bioss)


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    Structured Review

    Bioss primar antibodies against myeloperoxidase
    Primar Antibodies Against Myeloperoxidase, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mpo+polyclonal/MPO+Polyclonal+Antibody/pm42821166-48-5-11
    Average 94 stars, based on 32 article reviews
    primar antibodies against myeloperoxidase - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Clinical Proteomics:

    Article Title: Gasdermin D-dependent platelet pyroptosis exacerbates NET formation and inflammation in severe sepsis.
    Article Snippet: .. MPO–DNA complexes and dsDNA in plasma of human/mice and medium supernatant were detected by anti-MPO polyclonal (biotin conjugated) primary antibody (Bioss, 1:500, bs-4943R-Biotin) with a Cell Death Detection enzyme-linked immunosorbent assay (ELISA) kit (Roche, 11774425001)60 and Quant-iT PicoGreen dsDNA reagent (Invitrogen, catalog no. P7581) according to the manufacturer’s protocol. ..

    Article Title: GSDMD-dependent platelet pyroptosis exacerbates NET formation and inflammation in severe sepsis
    Article Snippet: 567 Measurement of NETs 568 To quantify NETs in plasma of human/mice and medium supernatant, dsDNA was 569 measured in severe sepsis using Quant-iT PicoGreen dsDNA reagent (Invitrogen, 570 P7581) according to the manufacturer's protocol. .. MPO-DNA complexes in plasma of 571 human/mice and medium supernatant were detected by anti-MPO polyclonal (Biotin 572 Conjugated) primary antibody (Bioss,bs-4943R-Biotin) and Cell Death Detection 573 ELISA kit (Roche, 11774425001) 53 . ..

    Article Title: Gasdermin D-dependent platelet pyroptosis exacerbates NET formation and inflammation in severe sepsis
    Article Snippet: Purified human PMNs (2 × 10 5 per ml) were prestimulated with PMA (50 nM; Sigma, catalog no. P1585) , that used as classical NET stimuli for subsequent assessments, resting platelets or pyroptotic platelets (1 μg ml −1 rhS100A8/A9-induced; BioLegend, catalog no. 753404) (0.04 × 10 5 per ml) for 4 h. To further confirm that pyroptotic platelets triggered NET formation, purified murine PMNs (2 × 10 5 per ml) were cocultured with resting WT platelets or with rmS100A8/A9-induced (1 μg ml −1 ; BioLegend, catalog no. 765506) Tlr4 −/− platelets (0.04 × 10 5 per ml) for 4 h. Use of S100A8/A9 was determined according to previous studies . .. MPO–DNA complexes and dsDNA in plasma of human/mice and medium supernatant were detected by anti-MPO polyclonal (biotin conjugated) primary antibody (Bioss, 1:500, bs-4943R-Biotin) with a Cell Death Detection enzyme-linked immunosorbent assay (ELISA) kit (Roche, 11774425001) and Quant-iT PicoGreen dsDNA reagent (Invitrogen, catalog no. P7581) according to the manufacturer’s protocol. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Gasdermin D-dependent platelet pyroptosis exacerbates NET formation and inflammation in severe sepsis.
    Article Snippet: .. MPO–DNA complexes and dsDNA in plasma of human/mice and medium supernatant were detected by anti-MPO polyclonal (biotin conjugated) primary antibody (Bioss, 1:500, bs-4943R-Biotin) with a Cell Death Detection enzyme-linked immunosorbent assay (ELISA) kit (Roche, 11774425001)60 and Quant-iT PicoGreen dsDNA reagent (Invitrogen, catalog no. P7581) according to the manufacturer’s protocol. ..

    Article Title: Gasdermin D-dependent platelet pyroptosis exacerbates NET formation and inflammation in severe sepsis.
    Article Snippet: Immunofluorescence: goat polyclonal anti-NLRP3 (Abcam, ab4207), rabbit polyclonal anti-ASC (Novusbio, NBP1-78978), rabbit monoclonal anti-CD41 (Abcam, ab134131), Alexa Fluor 488 Anti-mouse CD41 (BioLegend, 133908), Hoechst (Thermo, H3570), mouse monoclonal anti-myeloperoxidase (Genetx, gtx75318), rabbit monoclonal anti-myeloperoxidase (Abcam, ab208670) and rabbit polyclonal anti-Histone H3 (citrulline R2 + R8 + R17, Abcam, ab5103), Alexa Fluor 488-conjugated anti-goat IgG antibody (BioLegend, 405508), Alexa Fluor 647-conjugated anti-rabbit IgG secondary antibody (Abcam, ab150079), Alexa Fluor 594-conjugated anti-rabbit IgG secondary antibody (Abcam, ab150084), Alexa Fluor 594-conjugated anti-mouse IgG secondary antibody (Abcam, ab150116), Alexa Fluor 488-conjugated anti-goat IgG secondary antibody (Abcam, ab150113), Alexa Fluor 488-conjugated anti-rabbit IgG secondary antibody (Abcam, ab150077) and Alexa Fluor 488-conjugated anti-mouse IgG secondary antibody (Abcam, ab150113). .. ELISA: anti-MPO polyclonal (Biotin Conjugated) primary antibody (Bioss, bs-4943R-Biotin). .. WB: polyclonal rabbit anti-GSDMD (Cell Signaling Technology, 1:1000, #96458S), anti-NLRP3 (Cell Signaling Technology, #15101S), anti-TMS1/ASC (Abcam, ab151700), anti-pro Caspase 1 + p10 + p12 (Abcam, ab179515), -Actinin (Abcam, ab68194) and -Actin (Abcam,ab6276).

    Article Title: GSDMD-dependent platelet pyroptosis exacerbates NET formation and inflammation in severe sepsis
    Article Snippet: 567 Measurement of NETs 568 To quantify NETs in plasma of human/mice and medium supernatant, dsDNA was 569 measured in severe sepsis using Quant-iT PicoGreen dsDNA reagent (Invitrogen, 570 P7581) according to the manufacturer's protocol. .. MPO-DNA complexes in plasma of 571 human/mice and medium supernatant were detected by anti-MPO polyclonal (Biotin 572 Conjugated) primary antibody (Bioss,bs-4943R-Biotin) and Cell Death Detection 573 ELISA kit (Roche, 11774425001) 53 . ..

    Article Title: Gasdermin D-dependent platelet pyroptosis exacerbates NET formation and inflammation in severe sepsis
    Article Snippet: Purified human PMNs (2 × 10 5 per ml) were prestimulated with PMA (50 nM; Sigma, catalog no. P1585) , that used as classical NET stimuli for subsequent assessments, resting platelets or pyroptotic platelets (1 μg ml −1 rhS100A8/A9-induced; BioLegend, catalog no. 753404) (0.04 × 10 5 per ml) for 4 h. To further confirm that pyroptotic platelets triggered NET formation, purified murine PMNs (2 × 10 5 per ml) were cocultured with resting WT platelets or with rmS100A8/A9-induced (1 μg ml −1 ; BioLegend, catalog no. 765506) Tlr4 −/− platelets (0.04 × 10 5 per ml) for 4 h. Use of S100A8/A9 was determined according to previous studies . .. MPO–DNA complexes and dsDNA in plasma of human/mice and medium supernatant were detected by anti-MPO polyclonal (biotin conjugated) primary antibody (Bioss, 1:500, bs-4943R-Biotin) with a Cell Death Detection enzyme-linked immunosorbent assay (ELISA) kit (Roche, 11774425001) and Quant-iT PicoGreen dsDNA reagent (Invitrogen, catalog no. P7581) according to the manufacturer’s protocol. ..

    Article Title: Gasdermin D-dependent platelet pyroptosis exacerbates NET formation and inflammation in severe sepsis.
    Article Snippet: .. Anti-MPO polyclonal (Biotin Conjugated) primary antibody (Bioss, bs-4943R-Biotin) used for ELISA was validated for this application by the investigators in preceding study (Middleton, E.A., et al., Blood, 136, 1169-1179, 2020). .. The platelet-specific Gsdmd KO (Gsdmd^fl/fl PF4-Cre) mice (by crossing Gsdmd^fl/fl mice with PF4-Cre mice) and global S100a9 deficient mice (using CRISPR/Cas9) were designed and generated (Shanghai Model Organisms Center, China).



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